The Challenges of Biotherapeutic Development
Researchers within (bio)pharmaceutical sciences are increasingly tasked with gaining a deeper knowledge and understanding of the effects interactions and stressors such as pH or temperature, have on the conformational structure of biotherapeutic proteins. This is a very challenging task and researchers often use a variety of analytical tools to study the higher order structure of the biotherapeutics, monitor protein/protein-drug interactions and perform epitope mapping.
In recent years Hydrogen Deuterium Exchange Mass Spectrometry (HDX-MS) has emerged as a valuable technique within the arsenal of analytical tools available for providing such insights into protein structure conformation. However, until recently HDX-MS was confined mostly to academic research laboratories, due to a lack of automation and limited data interpretation tools, as well as the need to have reliable monoisotopic mass assignments.
The Bruker HDX Solution combines the LEAP HDx PAL, with the market-leading Bruker maXis II ETD and state of the art software from Sierra Analytics (HDExaminer) for data analysis. The industry-leading ETD capabilities of Bruker’s maXis II ETD UHR-QTOF offers unparalleled sequence coverage for middle-down and top-down studies while maintaining access to your existing peptide mapping workflows. However, the evolution of HDX-MS from bottom-up strategies based upon pepsin-digestion toward middle-down and top-down studies utilizing the power of ETD fragmentation has the potential to revolutionize the way conformational studies are performed.
The Bruker HDX Solution tool kit delivers automated and reliable HDX-MS workflows with correct monoisotopic accurate-mass readout to fully explore quantitative results from H/D ratios. This enables structural insights about biomolecules to be accessed reliably and with greater certainty and confidence.
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